Research TechnicianHillman Cancer Center seeks a qualified research technician to assist in transplant and leukemia immunotherapy research in Dr. Sawa Itos laboratory. The program aims to perform immunological correlating studies for the clinical trial and understand the treatment resistance mechanism. The technician is expected to perform human sample processing, experiments, data collection, and analysis of cellular, immunological, and molecular assays, including flow cytometry, primary cell and cell line culture, cell line development, DNA/RNA/protein preparation, RT-PCR and DNA/RNA sequencing, and T cell receptor TCR transduction to the human primary cells. The optimal candidate should have at least a Bachelors degree with 3 years of experience or a Masters degree with 1 year of experience. Experience or training in either molecular biology or immunology is preferred.We aim to conduct extensive correlating studies, including flow-based immunophenotyping assays and single-cell RNAseq, to assess leukemia IFN-gamma responsiveness. In addition to this new funding, we have an ongoing project to characterize the T cell receptor TCR clones of alloreactive T cells and discover the novel antigens recognized by these alloreactive TCRs. We seek a Research technician in our lab to accomplish these new and ongoing projects.Job SummaryPerforms a variety of independent and complex laboratory work and experiments. Analyzes and interprets data, compiles results, and prepares reports. Maintains inventory, ordering, and logs. Writes manuscripts and grant proposals and prepares data visualizations.Essential FunctionsCandidate must be able to independently perform advanced scientific experiments. Must be highly organized and detail oriented.Physical EffortModerate physical effort required. May be required to stand/move for several hours at a time. May be required to move objects up to 50 pounds with or without assistance of equipment.The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.Assignment Category: Full-time regular
Job Classification: Staff.Laboratory Research Technician IV
Job Family: Research
Job Sub Family: Laboratory
Campus: Pittsburgh
Minimum Education Level Required: Bachelors Degree
Minimum Years of Experience Required: 3
Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: Monday - Friday Work Arrangement: Monday - Friday
Hiring Range: $43,894.50 to $53,711.00
RelocationOffered: No
Visa Sponsorship Provided: No
Background Check: For position finalists, employment with the University will require successful completion of a background check
Child Protection Clearances: Not Applicable
Required Documents: Resume
Optional Documents: Cover LetterPI253945439