Job Title: Associate Scientist Location: Cambridge, MA Type: Contract Responsibilities
Conduct routine end-to-end molecular biology and biochemistry techniques including but not limited to:
Cell transformations, day cultures, bacterial cultures, plasmid purification and digestions.
DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS).
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing.
Transient cell culture techniques such as transfection, cell number and viability monitoring, cell lysis and protein harvest
Purification of proteins using liquid chromatography techniques.
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using Microsoft products and an online database for request management.
Maintaining reagent, DNA, RNA and protein inventory.
Effectively communicating with team members to meet strict schedules and deadlines.
Requirements
Qualifications:
Proficiency in biotechnology laboratory techniques and performing quantitative analysis
Experience working with bacterial cell lines and cell culture
Knowledge of nucleic acid purification and synthesis
Knowledge of protein purification techniques
Must have good practical skills in a laboratory setting such as keeping an organized laboratory notebook and maintaining laboratory cleanliness to minimize contamination
Ability to multitask and keep an organized record of daily work in an electronic lab notebook
Must be able to follow detailed protocols and weekly schedules
Must have the ability to concentrate while doing repetitive tasks
Must follow laboratory safety rules
Required Skills:
Proficiency in biotechnology laboratory techniques and performing quantitative analysis
Knowledge of nucleic acid purification and synthesis
Knowledge of protein purification techniques
Preferred Skills:
Experience working with bacterial cell lines and cell culture.
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing.
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
Education
B.Sc in biochemistry, chemistry, biological/life sciences
Associates, Certificate in Biotechnology
High School diploma with at least 2 years of relevant laboratory research school
Benefits
System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.
System One, and its subsidiaries including Joulé, ALTA IT Services, CM Access, TPGS, and MOUNTAIN, LTD., are leaders in delivering workforce solutions and integrated services across North America. We help clients get work done more efficiently and economically, without compromising quality. System One not only serves as a valued partner for our clients, but we offer eligible full-time employees health and welfare benefits coverage options including medical, dental, vision, spending accounts, life insurance, voluntary plans, as well as participation in a 401(k) plan.System One is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, age, national origin, disability, family care or medical leave status, genetic information, veteran status, marital status, or any other characteristic protected by applicable federal, state, or local law.