The Research Technician is a member of the Cell and Developmental Biology Department within Basic Sciences at Vanderbilt University and is a key individual contributor who will use single cell RNAseq data to investigate synapse formation in the nervous system of C. elegans. Reporting to the Principal Investigator, the Research Technician interacts with students, staff, and faculty within the laboratory.This position will provide an opportunity to learn methods for collecting and analyzing single cell RNAseq data (scRNA-seq), and molecular genetics and live cell imaging in the model organism, C. elegans. Activities: Collect and analyze scRNA-Seq data; Operate fluorescence microscopes to monitor GFP expression in nematode cultures; Maintain transgenic nematode strains and databases. About the Work Unit:The Department of Cell and Developmental Biology is a vibrant, interdisciplinary environment for cutting-edge research over a scale that spans seven powers of ten, from single molecules to whole organisms. The difference between a test tube and a cell is spatio-temporal organization, and we study molecular, cellular and tissue organization in many of the laboratories within our department, seeking insights into fundamental biological questions and human disease. This year we achieved the #3 ranking in the nation for funding, as compared with similar departments in research institutions and universities. We are an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top-ranked developmental biology program, and exceptionally strong faculty. Recent faculty recruits are studying cell migration using super-resolution microscopy, single molecule analysis of microtubule dynamics, systems biology of intestinal epithelia, stem cell biology, and the regulation of plasma membrane composition and aging.Key Functions and Expected Performance:
Learn methods for collecting and analyzing single cell RNA-Seq data
Use molecular genetics and live cell imaging to investigate the formation of synapses in the nervous system of C. elegans.
Works with Laboratory Manager to maintain freezers, prepare competent cells, ship nematode strains and reagents to other laboratories, order supplies and maintain equipment.
Prepares sterile media, pour nutrient agar plates, inoculate bacterial cultures as needed.
Supervisory Relationships:
This position does not have supervisory responsibility.
The position reports functionally and administratively to the Principal Investigator.
Education and Certifications:
A Bachelor’s degree in Biology or a related field from an accredited institution of higher education is necessary.
Experience and Skills:
Experience using computers and programming software, specifically python or R is preferred.
Experience with C. elegans is preferred
Experience with high resolution (confocal) fluorescence imaging is preferred.
Ability to maintain quality, safety, and/or infection control standards is preferred.
Ability to collect and process research data and accurately record findings is preferred.
Basic knowledge and understanding of scientific research methodology is preferred.
Ability to analyze and interpret statistical data is preferred.
Working knowledge of research techniques, instrumentation, and/or equipment, as specified in the research study is preferred.
Knowledge of data management techniques is preferred.
Commitment to Equity, Diversity, and Inclusion
At Vanderbilt University, we are intentional about and assume accountability for fostering advancement and respect for equity, diversity, and inclusion for all students, faculty, and staff. Our commitment to diversity makes us who we are. We have created a community that celebrates differences and lets individuality thrive. As part of this commitment, we actively value diversity in our workplace and learning environments as we seek to take advantage of the rich backgrounds and abilities of everyone. The diverse voices of Vanderbilt represent an invaluable resource for the University in its efforts to fulfill its mission and strive to be an example of excellence in higher education. Vanderbilt University is an equal opportunity, affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law. Please note, all candidates selected for an offer of employment are subject to pre-employment background checks, which may include but are not limited to, based on the role for which they have been selected: criminal history, education verification, social media review, motor vehicle records, credit history, and professional license verification.