Vacancy caducado!
SCIENTIST BOSTON, MA Required Skills:
- B.S/MS in Molecular Biology, Biochemistry, or related Biological Sciences with at least 2 years of laboratory experience.
- Strong technical background in molecular biology including cloning, vector engineering and in-vitro transcription.
- Experienced in bacterial and eukaryotic cell culture.
- The candidate must have expertise in molecular biology (cloning, Gibson assembly, PCR, Sanger sequencing), in-vitro transcription (IVT) and eukaryotic cell culture.
- Molecular biology skills including transformations and transfections.
- Western blotting(nice to have) and gel electrophoresis.
- Meticulous attention to detail, proficient reasoning and problem-solving skills in experimental data analysis and interpretation.
- Demonstrated ability to work effectively and productively in a team-oriented environment while maintaining a strong sense of integrity and confidentiality.
- Basic flow cytometry assay experience is a plus.
- Experience with luminescent or fluorescent reporter-based assays desirable.
- Previous work with RNA a plus.
- Mammalian cell culture experience is nice to have.
- Sequencing editing software.
- Basic MS office skills.
- We are seeking a highly motivated individual to join our client's highly innovative and multidisciplinary Cell Line Development team to support biologics drug development.
- The primary responsibility for this position will be to perform cloning, in-vitro transcription and in-vitro and cell-based characterization assays on transfected cells.
- Cloning, screening, sequencing, plasmid preparations and in-vitro transcription.
- Cell culture and transfections.
- Perform characterization assays post-transfection.
- Generation and characterization of protein expressing stable pools.
- Work independently to execute experiments, process data and analysis, and help develop SOPs.
- Efficient communication of results and proposing next steps to team members and project teams.
- Record all work and results in laboratory notebooks.
Vacancy caducado!